Guatemala, a Central American country south of Mexico, is distinguished by its steep volcanoes, vast rainforests and ancient Mayan sites.  A representative democracy, Guatemala's capital and largest city is Nueva Guatemala de la Asunción, also known as Guatemala City. The country's abundance of biologically significant and unique ecosystems, which includes a large number of endemic species, contributes to Mesoamerica's designation as a biodiversity hotspot. The country is also known for its rich and distinct culture, which is characterized by a fusion of Spanish and Indigenous influences.    

San Francisco de Quito, most often called Quito, is the capital city of Ecuador in northwestern South America. It is located in north-central Ecuador in the Guayllabamba river basin, on the eastern slopes of Pichincha, an active stratovolcano in the Andes mountains. With a population of 1,397,698 according to the last census (2001), and, as estimated by the municipality, approximately 1,504,991 in 2005, Quito is the second most populous city in Ecuador, after Guayaquil. It is also the capital of the Pichincha province and the seat of Metropolitan District of Quito. The canton recorded a population of 1,842,201 residents in the 2001 national census. In 2008 the city was designated as the headquarters of the Union of South American Nations.

San Juan, officially Municipio de la Ciudad Capital San Juan Bautista (Municipality of the Capital City, Saint John the Baptist), is the capital and most populous municipality in Puerto Rico, an unincorporated territory of the United States. As of the 2000 census, it has a population of 658,304 making it the 42nd-largest city under the jurisdiction of the United States. San Juan was founded by Spanish colonists in 1521, who called it Ciudad de Puerto Rico ("Rich Port City"). Puerto Rico's capital is the second oldest European-established city in the Americas, after Santo Domingo, in the Dominican Republic. Several historical buildings are located in San Juan; among the most notable are the city's former defensive forts, Fort San Felipe del Morro and Fort San Cristóbal, and La Fortaleza, the oldest executive mansion in continuous use in the Americas. 

Montevideo is the largest city, the capital, and the chief port of Uruguay. The settlement was established in 1726 by Bruno Mauricio de Zabala, as a strategic move amidst a Spanish-Portuguese dispute over the platine region, and as a counter to the Portuguese colony at Colonia del Sacramento. According to the census of 2004, Montevideo has a population of 1,325,968. It has an area of 200 square miles and extends 12 miles from west to east. The southernmost cosmopolitan capital city in the Americas and third most southern in the world, it is situated in the southern coast of the country, on the northeastern bank of the Río de la Plata. The city was under brief British rule in 1807 and was involved in the first major naval battle in the Second World War: the Battle of the River Plate.

Asunción is the capital and largest city of Paraguay. The Spanish word asunción means assumption in English. It refers to the Assumption of Mary; the full name means The Very Noble and Loyal City of Our Lady Saint Mary of the Assumption. The "Ciudad de Asunción" is an autonomous capital district not part of any department. The city of Asuncion is home to a little more than 500,000 people. It is a young city: 65% of its residents are under the age of 30. From Saturday in the evening and all Sunday most businesses are closed and the city centre can appear fairly deserted.

Bogotá is Colombia's beating heart – an interesting, beautiful and vibrant capital. It is the city of Colombia with the largest number of universities and research centers, and has an important cultural offer, which is represented in many museums, theaters and libraries. Bogotá is also known for its trendy nightlife.

Medellín is the mountain capital of Antioquia province, Colombia. It is a dynamic nucleus of the nation. The vibrant and tropical city is the second largest in the country behind Bogota and boasts an average annual temperature of 73 degrees Fahrenheit, which has earned it the epithet of City of Eternal Spring. Medellín is famous for its gardens, its flowers and a large variety of orchids. Today, it is one of the most interesting and innovative city to visit.

Guayaquil is the largest and the most populous city in Ecuador, with around 2.69 million people in the metropolitan area, as well as the nation's main port. It is one of the main South American ports in the Pacific. The climate is hot for the first semester of the year and breeze cool for the rest of it. A proud city of the tropics where foreigners are well received, music rules and seafood is a must. In the last decade, the city has undergone positive change. The beautiful riverfront promenade "Malecon 2000", green areas and modern shopping arcades all over the city attracts tourism from inside and outside the country.

Santiago de Cali, usually known by its short name "Cali", is a significant industrial and commercial center of activity in Colombia. It is one of the fastest growing economies in the country and is also known as ''The World's Capital of Salsa Music''. Cali is known for its wonderful weather with beautiful, warm and sunny days and is also famous for its historical places, entertainment and day and night activities.